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Real time PCR

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Techniques for detecting and amplifying nucleic acids are now extremely useful in biological research. These methods are used by researchers in a wide range of applications in the life sciences, including basic research, biotechnology, medicine, diagnostics, and more.

Real-time PCR can be used for both quantitative and qualitative analysis of nucleic acids in this field. Choosing the best method to use requires a comprehensive understanding of this technology. The Real-time PCR course will familiarize you with the technology’s basics, applications, implementation, analysis, and troubleshooting, and you will obtain great mastery and skill in applying this technique in your research and scientific path.

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Introduction to Primer Design and PCR

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Polymerase chain reaction (PCR) is a technique used to make millions of copies of a specific small amount of DNA in a laboratory. The technique was introduced in 1983 by an American biochemist named Karry Mullis, and for this valuable work he received the Nobel Prize in Chemistry in 1993.

In this course, you will learn the basics of primer design, both manually and using the appropriate software. This course is presented in such a way that even if you do not have sufficient knowledge about it, after participating in this course and a little practice, you will be able to design your primers with sufficient mastery, use PCR for various purposes, and have the ability to debug PCR.

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Recombinant protein expression: From beginner

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Microbial systems have revolutionized biochemistry due to their ability to produce recombinant proteins. We are fast approaching the end of the days when a given protein could be purified with kilograms of plant or animal tissue or large amounts of biological fluid. In any new research project requiring purified proteins, researchers immediately consider recombinant forms of those proteins. It allows the biochemical characterization, use in industrial processes, and development of commercial products when a recombinant protein can be expressed and purified in large quantities.

In order to express recombinant proteins in bacteria, DNA fragments (open reading frames, ORFs) need to be inserted into an expression vector, routinely a plasmid, and this vector needs to be transferred into bacterial cells (transformation). The cells are then cultured and induced to produce recombinant proteins.

This online course will teach you how to use the microbial expression system (Bacteria: pET) for your recombinant protein expression. Learn about the bacterial manipulation to improve protein production.

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Techniques in Molecular cloning

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The process of molecular cloning involves separating DNA sequences from any organism and placing them into a vector without altering their original sequence for amplification. Clones can be used to make large copies of DNA fragments that are intended for sequencing genes or expressing proteins to study protein function and expression. They can also be genetically altered in the laboratory to change their expression and function. Generating recombinant DNA, called Molecular Cloning, has affected all branches of biology. Gene cloning has become a standard procedure in a wide range of molecular biology laboratories.

With the advent of various molecular cloning technologies, such as the incorporation of DNA fragments and their transfer to bacteria in less than two hours, or the use of alternative gene cassettes, this branch of science has gained maximum flexibility and speed. In the near future, the disciplines of molecular cloning and biological synthetics will gain capabilities in the chemical manufacture of any specified DNA construct in vitro. These breakthroughs allow for speedier DNA structure and clone synthesis, as well as the development of gene therapy vectors, recombinant protein production techniques, and novel vaccinations.